Flow injection determination of vitamin K3 by a photoinduced chemiluminescent reaction

Abstract

The determination of vitamin K₃ using the coupling between a photochemical reaction and a chemiluminescent reaction in a flow system is described. The method is based on the photooxidation of ethanol sensitized by vitamin K₃ to yield hydrogen peroxide, which is monitored through the chemiluminescent reaction with luminol catalysed by hematin. The new approach allows the determination of vitamin K₃ in a wide concentration range (1 × 10^–7–5 × 10^–4 mol l^–1) with a throughput of 30 samples h^–1. The applicability of the method was demonstrated by the determination of vitamin K₃ in pharmaceutical preparations.