From the journal:

Organic & Biomolecular Chemistry

Facile DNA chemical ligation under mild conditions enabled by 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP)†

Hiroki Yamada, ORCID logo a   Yasuaki Kimura, ORCID logo b   Hiroshi Abe ORCID logo bcde  and  Junichiro Yamamoto ORCID logo *f  

* Corresponding authors

a Innovation Center, Research Division, Kyowa Kirin Co., Ltd, 3-6-6 Asahi, Machida, Tokyo 194-8533, Japan

b Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan

c Research Center for Materials Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan

d CREST, Japan Science and Technology Agency, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan

e Institute for Glyco-core Research (iGCORE), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan

f Research Planning Department, Research Division, Kyowa Kirin Co., Ltd, 3-6-6 Asahi, Machida, Tokyo 194-8533, Japan
E-mail: junichiro.yamamoto.9k@kyowakirin.com

Abstract

We report the chemical ligation of DNA using 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP). CDAP promoted DNA ligation without depurination or undesired side reactions to the nucleobase. Ligation of 3′-phosphate and 5′-hydroxyl termini was achieved at low temperatures with good yield. This study provides a novel method for the synthesis of artificial oligonucleotides.

Graphical abstract: Facile DNA chemical ligation under mild conditions enabled by 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP)

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Article information

DOI
https://doi.org/10.1039/D5OB00411J
Article type
Communication
Submitted
06 Mar 2025
Accepted
16 Apr 2025
First published
17 Apr 2025

Org. Biomol. Chem., 2025, Advance Article

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Facile DNA chemical ligation under mild conditions enabled by 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP)

H. Yamada, Y. Kimura, H. Abe and J. Yamamoto, Org. Biomol. Chem., 2025, Advance Article , DOI: 10.1039/D5OB00411J

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