A sensitive dual-signal electrochemiluminescence immunosensor based on Ru(bpy)32+@HKUST-1 and Ce2Sn2O7 for detecting the heart failure biomarker NT-proBNP

Abstract

A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy)₃²⁺@HKUST-1/TPA and Ce₂Sn₂O₇/K₂S₂O₈ probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy)₃²⁺, effectively improving the anodic signal intensity, while the emerging Ce₂Sn₂O₇ emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy. This dual-signal immunosensor has a wide linear range (5 × 10⁻⁴–1 × 10⁴ ng mL⁻¹) and a low quantitative detection limit, simultaneously showing high sensitivity, stability and reproducibility, as well as the detection capability of actual serum samples. This dual signal-calibrated immunoassay platform not only reduces the false positive rate of detection results but also provides a promising method for the early diagnosis of heart failure.