Issue 15, 2023

Flow bioprocessing of citrus glycosides for high-value aglycone preparation

Abstract

An efficient one-pot, 2-step flow bioprocess for the hydrolysis of hesperidin (HES) and rutin (RT), citrus rutinosyl flavonoids, has been developed for the obtainment of the corresponding aglycones. A commercially available α-rhamnosidase (RN) and an extremophilic home-made β-glycosidase (HOR) have been co-immobilized on glyoxyl-agarose beads to prepare a high-performing multi-active biocatalyst (imm-RN–HOR). After the optimization of the reaction conditions in batch mode, a “flow switch” was applied, increasing the productivity (>99% m.c., 5 min), cost-efficiency and sustainability of the overall process. Due to the recovery and reuse of all the materials involved in the flow biotransformation, this strategy is effectively a zero-waste process.

Graphical abstract: Flow bioprocessing of citrus glycosides for high-value aglycone preparation

Supplementary files

Article information

Article type
Communication
Submitted
30 Apr 2023
Accepted
20 Jun 2023
First published
28 Jun 2023
This article is Open Access
Creative Commons BY license

Catal. Sci. Technol., 2023,13, 4348-4352

Flow bioprocessing of citrus glycosides for high-value aglycone preparation

A. Colacicco, G. Catinella, C. Pinna, A. Pellis, S. Farris, L. Tamborini, S. Dallavalle, F. Molinari, M. L. Contente and A. Pinto, Catal. Sci. Technol., 2023, 13, 4348 DOI: 10.1039/D3CY00603D

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