Issue 85, 2023

Unusual topological RNA G-quadruplex formed by an RNA duplex: implications for the dimerization of SARS-CoV-2 RNA

Abstract

The infectious disease coronavirus 2019 (SARS-CoV-2) is caused by a virus that has RNA as its genetic material. To understand the detailed structural features of SARS-COV-2 RNA, we probed the RNA structure by NMR. Two RNA sequences form a duplex and self-associate to form a dimeric G-quadruplex. The FrG nucleoside was employed as a 19F sensor to confirm the RNA structure in cells by 19F NMR. A FRET assay further demonstrated that the dimeric G-quadruplex resulted in RNA dimerization in cells. These results provide the basis for the elucidation of SARS-COV-2 RNA function, which provides new insights into developing novel antiviral drugs against SARS-COV-2.

Graphical abstract: Unusual topological RNA G-quadruplex formed by an RNA duplex: implications for the dimerization of SARS-CoV-2 RNA

Supplementary files

Article information

Article type
Communication
Submitted
03 Jul 2023
Accepted
20 Sep 2023
First published
11 Oct 2023
This article is Open Access
Creative Commons BY license

Chem. Commun., 2023,59, 12703-12706

Unusual topological RNA G-quadruplex formed by an RNA duplex: implications for the dimerization of SARS-CoV-2 RNA

S. Wang, Y. Song, Z. He, H. Saneyoshi, R. Iwakiri, P. Xu, C. Zhao, X. Qu and Y. Xu, Chem. Commun., 2023, 59, 12703 DOI: 10.1039/D3CC03192F

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