Issue 53, 2023

Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis

Abstract

Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes.

Graphical abstract: Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis

Supplementary files

Article information

Article type
Communication
Submitted
04 Apr 2023
Accepted
07 Jun 2023
First published
08 Jun 2023

Chem. Commun., 2023,59, 8234-8237

Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis

Y. T. C. Ho, J. A. Kaczmarski, J. Tailhades, T. Izoré, D. L. Steer, R. B. Schittenhelm, M. Tosin, C. J. Jackson and M. J. Cryle, Chem. Commun., 2023, 59, 8234 DOI: 10.1039/D3CC01578E

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