Issue 21, 2022

A dual-emission fluorescence-enhanced probe for hydrogen sulfide and its application in biological imaging

Abstract

Hydrogen sulfide (H2S) is an emerging gasotransmitter in the regulation of numerous physiological and pathological processes. Herein, we reported a dual-emission fluorescence-enhanced probe DCH-S for the detection of biological H2S. The probe is composed of a coumarin-SBD hybrid and a recognition group of 2,4-dinitrobenzenesulfonate (DNBS). In response to H2S, DCH-S rapidly changed into DCH with elimination of the DNBS group and then exhibited a fluorescence enhancement at both 456 nm and 600 nm. The fluorescence intensities reached a plateau within 5 min and the limit of detection was calculated as 47 nM. DCH-S showed high selectivity and anti-interference ability to H2S over other related analytes. DCH-S was also found to function efficiently under a wide pH range of 5–9 and display low toxicity to living MCF-7 cells. More importantly, DCH-S was successfully applied to detect exogenous/endogenous H2S in living cells and zebrafish via dual-channel imaging.

Graphical abstract: A dual-emission fluorescence-enhanced probe for hydrogen sulfide and its application in biological imaging

Supplementary files

Article information

Article type
Paper
Submitted
10 Mar 2022
Accepted
01 May 2022
First published
13 May 2022

New J. Chem., 2022,46, 10184-10190

A dual-emission fluorescence-enhanced probe for hydrogen sulfide and its application in biological imaging

M. Li, Y. Jiao and C. Duan, New J. Chem., 2022, 46, 10184 DOI: 10.1039/D2NJ01195F

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