Orthogonal protein decoration of DNA nanostructures based on SpyCatcher–SpyTag interaction†
Sandra
Kröll, 
a
Leonie
Schneider,
a
Parvesh
Wadhwani,
b
Kersten S.
Rabe
a
and
Christof M.
Niemeyer
*a
a
Leonie
Schneider,
a
Parvesh
Wadhwani,
b
Kersten S.
Rabe
a
and
Christof M.
Niemeyer
*a
Abstract
We present an efficient and readily applicable strategy for the covalent ligation of proteins to DNA origami by using the SpyCatcher–SpyTag (SC–ST) connector system. This approach showed orthogonality with other covalent connectors and has been used exemplarily for the immobilization and study of stereoselective ketoreductases to gain insight into the spatial arrangement of enzymes on DNA nanostructures.
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