Issue 97, 2022
From the journal:

Chemical Communications

A functional RNA/DNA circuit for one-pot detection of SARS-CoV-2 RNA†

Dayong Li,a   Wenting Cheng,a   Zhiqiang Hou,a   Chengjie Duan,a   Yanheng Yao,a   Yan Chen,a   Gang Yang,*b   Zhouxiang Cheng*c  and  Yang Xiang ORCID logo *ad  

* Corresponding authors

a State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China
E-mail: xiangy@nju.edu.cn

b Pulmonary and Critical Care Department, Wuhu Hospital of East China Normal University, Wuhu 241000, P. R. China
E-mail: 13855306767@163.com

c Center for Disease Control and Prevention, Wuhu 241000, P. R. China
E-mail: czx6404@126.com

d State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, P. R. China

Abstract

A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a functional RNA/DNA circuit. By ingeniously integrating the nucleic acid circuit technology and CRISPR/cas12a system, this method can achieve femtomolar detection of the target RNA in one step and successfully distinguish COVID-19 positive cases from clinical samples, proving its great potential for clinical application.

Graphical abstract: A functional RNA/DNA circuit for one-pot detection of SARS-CoV-2 RNA

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Article information

DOI
https://doi.org/10.1039/D2CC05251B
Article type
Communication
Submitted
24 Sep 2022
Accepted
08 Nov 2022
First published
09 Nov 2022

Chem. Commun., 2022,58, 13475-13478

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A functional RNA/DNA circuit for one-pot detection of SARS-CoV-2 RNA

D. Li, W. Cheng, Z. Hou, C. Duan, Y. Yao, Y. Chen, G. Yang, Z. Cheng and Y. Xiang, Chem. Commun., 2022, 58, 13475 DOI: 10.1039/D2CC05251B

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