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Issue 23, 2021
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Quantification and molecular imaging of fatty acid isomers from complex biological samples by mass spectrometry

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Abstract

Elucidating the isomeric structure of free fatty acids (FAs) in biological samples is essential to comprehend their biological functions in various physiological and pathological processes. Herein, we report a novel approach of using peracetic acid (PAA) induced epoxidation coupled with mass spectrometry (MS) for localization of the C[double bond, length as m-dash]C bond in unsaturated FAs, which enables both quantification and spatial visualization of FA isomers from biological samples. Abundant diagnostic fragment ions indicative of the C[double bond, length as m-dash]C positions were produced upon fragmentation of the FA epoxides derived from either in-solution or on-tissue PAA epoxidation of free FAs. The performance of the proposed approach was evaluated by analysis of FAs in human cell lines as well as mapping the FA isomers from cancer tissue samples with MALDI-TOF/TOF-MS. Merits of the newly developed method include high sensitivity, simplicity, high reaction efficiency, and capability of spatial characterization of FA isomers in tissue samples.

Graphical abstract: Quantification and molecular imaging of fatty acid isomers from complex biological samples by mass spectrometry

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Supplementary files

Article information


Submitted
20 Mar 2021
Accepted
04 May 2021
First published
04 May 2021

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2021,12, 8115-8122
Article type
Edge Article

Quantification and molecular imaging of fatty acid isomers from complex biological samples by mass spectrometry

H. Zhang, M. Xu, X. Shi, Y. Liu, Z. Li, J. C. Jagodinsky, M. Ma, N. V. Welham, Z. S. Morris and L. Li, Chem. Sci., 2021, 12, 8115
DOI: 10.1039/D1SC01614H

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