Issue 23, 2021

Sample-to-analysis platform for rapid intracellular mass spectrometry from small numbers of cells

Abstract

Real-time, advanced diagnostics of the biochemical state within cells remains a significant challenge for research and development, production, and application of cell-based therapies. The fundamental biochemical processes and mechanisms of action of such advanced therapies are still largely unknown, including the critical quality attributes that correlate to therapeutic function, performance, and potency and the critical process parameters that impact quality throughout cell therapy manufacturing. An integrated microfluidic platform has been developed for in-line analysis of a small number of cells via direct infusion nano-electrospray ionization mass spectrometry. Central to this platform is a microfabricated cell processing device that prepares cells from limited sample volumes removed directly from cell culture systems. The sample-to-analysis workflow overcomes the labor intensive, time-consuming, and destructive nature of existing mass spectrometry approaches for analysis of cells. By providing rapid, high-throughput analyses of the intracellular state, this platform enables untargeted discovery of critical quality attributes and their real-time, in-process monitoring.

Graphical abstract: Sample-to-analysis platform for rapid intracellular mass spectrometry from small numbers of cells

Supplementary files

Article information

Article type
Paper
Submitted
30 Sep 2021
Accepted
25 Oct 2021
First published
01 Nov 2021

Lab Chip, 2021,21, 4696-4706

Sample-to-analysis platform for rapid intracellular mass spectrometry from small numbers of cells

A. L. Culberson, M. A. Chilmonczyk, P. A. Kottke, A. C. Bowles-Welch, D. Ghoshal and A. G. Fedorov, Lab Chip, 2021, 21, 4696 DOI: 10.1039/D1LC00884F

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