Issue 23, 2021

Fully automated light transmission aggregometry on a disc for platelet function tests

Abstract

Platelet function tests, a group of assays that measure the ability of platelets to aggregate and promote clotting in a sample of blood, are performed in various medical fields to assess inherited platelet function disorders and monitor antiplatelet therapies. Light transmission aggregometry (LTA) is considered the gold standard for platelet function assessment. However, the lack of a standardized protocol is a major drawback when applied at the point of care. Moreover, it is a time-consuming and labor-intensive assay that requires a large volume of blood. Here, we describe the design, fabrication, and operation of a centrifugal microfluidic disc that can perform a fully automated LTA assay from a small volume of a whole blood sample (<1 mL), achieving highly reproducible results (3.2% coefficient of variation) within a short period (<25 min). The assays performed with this device yield more precise and accurate results than traditional LTA because of the automation of the reaction steps, minimal human operation, robust detection strategy via the distinctive structure of the microfluidic chamber, and quick analysis that minimizes the adverse effects of platelet instability.

Graphical abstract: Fully automated light transmission aggregometry on a disc for platelet function tests

Supplementary files

Article information

Article type
Paper
Submitted
11 Aug 2021
Accepted
27 Oct 2021
First published
27 Oct 2021

Lab Chip, 2021,21, 4707-4715

Fully automated light transmission aggregometry on a disc for platelet function tests

C. Kim, J. Kim, J. Sabaté del Río, D. Y. Ki, J. Kim and Y. Cho, Lab Chip, 2021, 21, 4707 DOI: 10.1039/D1LC00708D

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