Issue 8, 2021

Separation, characterization and hypoglycemic activity in vitro evaluation of a low molecular weight heteropolysaccharide from the fruiting body of Phellinus pini

Abstract

Edible mushrooms have potential in anti-diabetic phytotherapy. They are rich in natural compounds such as polysaccharides, which have been known to have antihyperlipidemic effects since ancient times. A polysaccharide fraction of PP80 and a contained low molecular-weight (Mw), water-soluble polysaccharide (PPW-1, Mw: 3.2 kDa) were isolated from the fruiting body of Phellinus pini. Both PP80 and PPW-1 possess α-glucosidase inhibition and glucose consumption amelioration in an insulin-resistant HepG2 cell model. The α-glucosidase inhibitory activity of PPW-1 (IC50 = 2.2 ± 0.1 mg mL−1) is significantly (P < 0.01) higher than those of PP80 (IC50 = 13.1 ± 0.5 mg mL−1) and acarbose (IC50 = 4.3 ± 0.2 mg mL−1), behaving in a non-competitive inhibition manner. The structural characterization results indicated that PPW-1 is a homogeneous heteropolysaccharide composed of D-glucose, D-mannose, D-galactose and L-rhamnose. The major backbone of PPW-1 is primarily comprised of 1,6-linked glucopyranose, every third residue of which is branched at the O-3 position by a side chain consisting of 1,3-linked and terminal glucopyranose. In addition, small amounts of 1,2-linked-α-D-Manp, 1,6-linked-3-O-Me-α-D-Galp and rhamnose exist in PPW-1. In summary, PPW-1 is a novel heteropolysaccharide with potent in vitro hypoglycemic activity, and it may be a potential dietary component for improving glucose homeostasis.

Graphical abstract: Separation, characterization and hypoglycemic activity in vitro evaluation of a low molecular weight heteropolysaccharide from the fruiting body of Phellinus pini

Supplementary files

Article information

Article type
Paper
Submitted
29 Jan 2021
Accepted
18 Mar 2021
First published
19 Mar 2021

Food Funct., 2021,12, 3493-3503

Separation, characterization and hypoglycemic activity in vitro evaluation of a low molecular weight heteropolysaccharide from the fruiting body of Phellinus pini

K. Yang, Y. Jin, M. Cai, P. He, B. Tian, R. Guan, G. Yu and P. Sun, Food Funct., 2021, 12, 3493 DOI: 10.1039/D1FO00297J

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