Electroenzymatic choline sensing at near the theoretical performance limit

Abstract

A high performance, electroenzymatic microsensor for choline based on choline oxidase (ChOx) immobilized on Pt coated with permselective polymer layers has been created that exhibits sensitivity approaching the theoretical performance limit. Sensor construction was guided by simulations performed with a detailed mathematical model. Implantable microsensors with an array of electroenzymatic sensing sites provide a means to record concentration changes of choline, an effective surrogate for acetylcholine due to its very rapid turnover in the brain, and other neurochemicals in vivo. However, electroenzymatic sensors generally have insufficient sensitivity and response time to monitor neurotransmitter signaling on the millisecond timescale with cellular-level spatial resolution. Model simulations suggested that choline sensor performance can be improved significantly by optimizing immobilized ChOx layer thickness and minimizing the thicknesses of permselective polymer coatings as well. Electroenzymatic choline sensors constructed with a ∼5 μm-thick crosslinked ChOx layer atop 200 nm-thick permselective films (poly(m-phenylenediamine) and Nafion) exhibited unprecedented sensitivity and response time of 660 ± 40 nA μM⁻¹ cm⁻² at 37 °C and 0.36 ± 0.05 s, respectively, while maintaining excellent selectivity. Such performance characteristics provide greater flexibility in the design of microelectrode array (MEA) probes with near cellular-scale sensing sites arranged in more dense arrays. Also, faster response times enable better resolution of transient acetylcholine signals and better correlation of these events with electrophysiological recordings so as to advance study of brain function.