Visualization of lipophagy using a supramolecular FRET pair†
A rationally designed supramolecular FRET pair consisting of cyanine3-cucurbituril (Cy3-CB) and boron-dipyrromethene 630/650-adamantylammonium (BDP-AdA) can be used to visualize organelle-specific autophagy events. The intracellular accumulations of Cy3-CB in lysosomes and BDP-AdA in lipid droplets (LDs) and the formation of an intracellular host–guest complex between Cy3-CB and BDP-AdA resulting in FRET signals allow us to visualize the fusion of LDs with lysosomes, namely, lipophagy. This study demonstrates the potential of supramolecular imaging based on bio-orthogonal host–guest interactions in the investigation of selective autophagy events.
- This article is part of the themed collection: Host-Guest Chemistry