Issue 33, 2020

Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

Abstract

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

Graphical abstract: Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

Supplementary files

Article information

Article type
Edge Article
Submitted
30 Apr 2020
Accepted
07 Aug 2020
First published
10 Aug 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2020,11, 8955-8960

Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

A. T. Frawley, V. Wycisk, Y. Xiong, S. Galiani, E. Sezgin, I. Urbančič, A. Vargas Jentzsch, K. G. Leslie, C. Eggeling and H. L. Anderson, Chem. Sci., 2020, 11, 8955 DOI: 10.1039/D0SC02447C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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