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Issue 33, 2020
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Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

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Abstract

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

Graphical abstract: Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

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Supplementary files

Article information


Submitted
30 Apr 2020
Accepted
07 Aug 2020
First published
10 Aug 2020

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2020,11, 8955-8960
Article type
Edge Article

Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

A. T. Frawley, V. Wycisk, Y. Xiong, S. Galiani, E. Sezgin, I. Urbančič, A. Vargas Jentzsch, K. G. Leslie, C. Eggeling and H. L. Anderson, Chem. Sci., 2020, 11, 8955
DOI: 10.1039/D0SC02447C

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