Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Round, round we go – strategies for enzymatic cofactor regeneration

Author affiliations

Abstract

Covering: up to the beginning of 2020

Enzymes depending on cofactors are essential in many biosynthetic pathways of natural products. They are often involved in key steps: catalytic conversions that are difficult to achieve purely with synthetic organic chemistry. Hence, cofactor-dependent enzymes have great potential for biocatalysis, on the condition that a corresponding cofactor regeneration system is available. For some cofactors, these regeneration systems require multiple steps; such complex enzyme cascades/multi-enzyme systems are (still) challenging for in vitro biocatalysis. Further, artificial cofactor analogues have been synthesised that are more stable, show an altered reaction range, or act as inhibitors. The development of bio-orthogonal systems that can be used for the production of modified natural products in vivo is an ongoing challenge. In light of the recent progress in this field, this review aims to provide an overview of general strategies involving enzyme cofactors, cofactor analogues, and regeneration systems; highlighting the current possibilities for application of enzymes using some of the most common cofactors.

Graphical abstract: Round, round we go – strategies for enzymatic cofactor regeneration

Back to tab navigation

Article information


Submitted
31 Jan 2020
First published
25 Jun 2020

Nat. Prod. Rep., 2020, Advance Article
Article type
Review Article

Round, round we go – strategies for enzymatic cofactor regeneration

S. Mordhorst and J. N. Andexer, Nat. Prod. Rep., 2020, Advance Article , DOI: 10.1039/D0NP00004C

Social activity

Search articles by author

Spotlight

Advertisements