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Issue 9, 2020
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The quantum dot-FRET-based detection of vitamin B12 at a picomolar level

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Abstract

Herein we report the picomolar level detection of vitamin B12 (VB12) using orange-red emitting ligand-free Mn2+-doped ZnS quantum dots (QDs; λem = 587 nm) in an aqueous dispersion. Sensing was achieved following the quenching of the luminescence of the Mn2+-doped ZnS QDs with an increasing concentration of VB12. The Stern–Volmer constant was determined to be 5.2 × 1010 M−1. Importantly, the Mn2+-doped ZnS QDs exhibited high sensitivity towards VB12, with a limit of detection as low as 1.15 ± 0.06 pM (in the linear range of 4.9–29.4 pM) and high selectivity in the presence of interfering amino acids, metal ions, and proteins. Notably, a Förster resonance energy transfer (FRET) mechanism was primarily proposed for the observed quenching of luminescence of Mn2+-doped ZnS QDs upon the addition of VB12. The Förster distance (Ro) and energy transfer efficiency (E) were calculated to be 2.33 nm and 79.3%, respectively. Moreover, the presented QD-FRET-based detection may bring about new avenues for future biosensing applications.

Graphical abstract: The quantum dot-FRET-based detection of vitamin B12 at a picomolar level

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Article information


Submitted
29 Jun 2020
Accepted
10 Jul 2020
First published
13 Jul 2020

This article is Open Access

Nanoscale Adv., 2020,2, 3809-3814
Article type
Communication

The quantum dot-FRET-based detection of vitamin B12 at a picomolar level

S. Pramanik, S. Roy and S. Bhandari, Nanoscale Adv., 2020, 2, 3809
DOI: 10.1039/D0NA00540A

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