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Issue 3, 2020
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Monitoring protein-metal binding by 19F NMR – a case study with the New Delhi metallo-β-lactamase 1

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Abstract

19F NMR protein observed spectroscopy is evaluated as a method for analysing protein metal binding using the New Delhi metallo-β-lactamase 1. The results imply 19F NMR is useful for analysis of different metallated protein states and investigations on equilibrium states in the presence of inhibitors. One limitation is that 19F labelling may affect metal ion binding. The sensitive readout of changes in protein behaviour observed by 19F NMR spectra coupled with the broad scope of tolerated conditions (e.g. buffer variations) means 19F NMR should be further investigated for studying metal ion interactions and the inhibition of metallo-enzymes during drug discovery.

Graphical abstract: Monitoring protein-metal binding by 19F NMR – a case study with the New Delhi metallo-β-lactamase 1

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Supplementary files

Article information


Submitted
27 Aug 2019
Accepted
31 Dec 2019
First published
21 Feb 2020

This article is Open Access

RSC Med. Chem., 2020,11, 387-391
Article type
Research Article

Monitoring protein-metal binding by 19F NMR – a case study with the New Delhi metallo-β-lactamase 1

A. M. Rydzik, J. Brem, S. A. Chandler, J. L. P. Benesch, T. D. W. Claridge and C. J. Schofield, RSC Med. Chem., 2020, 11, 387
DOI: 10.1039/C9MD00416E

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