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Microfluidic device to study flow-free chemotaxis of swimming cells

Abstract

Microfluidic devices have been used in the last two decades to study in-vitro cell chemotaxis, but few existing devices generate gradients in flow-free conditions. Flow can bias cell directionality of adherent cells and precludes the study of swimming cell like naïve T lymphocytes, which only migrate in a non-adherent fashion. We developed two devices that create stable, flow-free, diffusion-based gradients and are adapted for adherent and swimming cells. The flow-free environment is achieved by using agarose gel barriers between a central channel with cells and side channels with chemoattractants. These barriers insulate cells from injection/rinsing cycles of chemoattractants, they dampen residual drift across the device, and they allow co-cultures of cells without physical interactions to study contactless paracrine communication. Our devices were used here to investigate neutrophil and naïve T lymphocyte chemotaxis.

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Supplementary files

Article information


Submitted
14 Jan 2020
Accepted
19 Mar 2020
First published
24 Mar 2020

Lab Chip, 2020, Accepted Manuscript
Article type
Paper

Microfluidic device to study flow-free chemotaxis of swimming cells

N. Garcia-Seyda, L. Aoun, V. Tishkova, V. Seveau de Noray, M. Biarnes-Pelicot, M. Bajenoff, M. Valignat and O. Theodoly, Lab Chip, 2020, Accepted Manuscript , DOI: 10.1039/D0LC00045K

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