Jump to main content
Jump to site search

Microfluidic device to study flow-free chemotaxis of swimming cells


Microfluidic devices have been used in the last two decades to study in-vitro cell chemotaxis, but few existing devices generate gradients in flow-free conditions. Flow can bias cell directionality of adherent cells and precludes the study of swimming cell like naïve T lymphocytes, which only migrate in a non-adherent fashion. We developed two devices that create stable, flow-free, diffusion-based gradients and are adapted for adherent and swimming cells. The flow-free environment is achieved by using agarose gel barriers between a central channel with cells and side channels with chemoattractants. These barriers insulate cells from injection/rinsing cycles of chemoattractants, they dampen residual drift across the device, and they allow co-cultures of cells without physical interactions to study contactless paracrine communication. Our devices were used here to investigate neutrophil and naïve T lymphocyte chemotaxis.

Back to tab navigation

Supplementary files

Article information

14 Jan 2020
19 Mar 2020
First published
24 Mar 2020

Lab Chip, 2020, Accepted Manuscript
Article type

Microfluidic device to study flow-free chemotaxis of swimming cells

N. Garcia-Seyda, L. Aoun, V. Tishkova, V. Seveau de Noray, M. Biarnes-Pelicot, M. Bajenoff, M. Valignat and O. Theodoly, Lab Chip, 2020, Accepted Manuscript , DOI: 10.1039/D0LC00045K

Social activity

Search articles by author