Issue 69, 2020
From the journal:

Chemical Communications

FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants†

Junyan Wang,ab   Tao Bing, ORCID logo *ab   Nan Zhang,ab   Xiangjun Liu ORCID logo ab  and  Dihua Shangguan ORCID logo *ab  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS, Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
E-mail: bingtao@iccas.ac.cn, sgdh@iccas.ac.cn

b University of the Chinese Academy of Sciences, Beijing 100049, China

Abstract

Herein, we report a FnCas12a/crRNA assisted Dumbbell-PCR method for the detection of isomiRs with double specificity and magnification. The single nucleotide variant of isomiRs in terminals and/or inner sequence could be discriminated by this strategy. Using this method, let-7a isomiRs in MCF-7 and MCF-7R cell lines were analyzed.

Graphical abstract: FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants

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Article information

DOI
https://doi.org/10.1039/D0CC04348F
Article type
Communication
Submitted
23 Jun 2020
Accepted
22 Jul 2020
First published
24 Jul 2020

Chem. Commun., 2020,56, 10038-10041

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FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants

J. Wang, T. Bing, N. Zhang, X. Liu and D. Shangguan, Chem. Commun., 2020, 56, 10038 DOI: 10.1039/D0CC04348F

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