Natural-abundance 17O NMR spectroscopy of magnetically aligned lipid nanodiscs

Abstract

Natural-abundance ¹⁷O NMR experiments are used to investigate the hydrated water in magnetically aligned synthetic polymer based lipid-nanodiscs. Residual quadrupole couplings (RQCs) measured from the observed five ¹⁷O (central and satellite) transitions, and molecular dynamics simulations, are used to probe the ordering of water molecules across the lipid bilayer.