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Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

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Abstract

Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.

Graphical abstract: Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

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Article information


Submitted
06 Mar 2020
Accepted
14 May 2020
First published
14 May 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

B. Krishnarjuna, T. Ravula and A. Ramamoorthy, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC01737J

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