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Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

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Abstract

Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.

Graphical abstract: Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

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Article information


Submitted
21 Jan 2020
Accepted
12 Mar 2020
First published
12 Mar 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

B. J. Bowen, A. R. McGarrity, J. A. Szeto, C. R. Pudney and D. D. Jones, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC00596G

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