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Issue 27, 2020
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The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi–bi mechanism

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Abstract

Eukaryotic tRNA-guanine transglycosylase (TGT) – an enzyme recently recognised to be of potential therapeutic importance – catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with 4 amino acids via a distinct mechanism to that reported for its eubacterial homologue. The presence of queuine is unequivocally required as a trigger for reaction between the enzyme and tRNA and exhibits cooperativity not seen using guanine as a substrate.

Graphical abstract: The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi–bi mechanism

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Article information


Submitted
23 Dec 2019
Accepted
29 Feb 2020
First published
09 Mar 2020

Chem. Commun., 2020,56, 3915-3918
Article type
Communication

The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi–bi mechanism

M. A. Alqasem, C. Fergus, J. M. Southern, S. J. Connon and V. P. Kelly, Chem. Commun., 2020, 56, 3915
DOI: 10.1039/C9CC09887A

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