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Efficient Cellular Uptake of Click Nucleic Acid Modified Proteins

Abstract

Efficient intracellular delivery of biomacromolecules such as proteins continues to remain a challenge despite its potential for medicine. In this work, we show that mScarlet, a non cytotoxic red fluorescent protein (RFP) conjugated to Click Nucleic Acids (CNA), a synthetic analog of DNA, undergo cell uptake significantly more than either native proteins or proteins conjugated with similar amounts of DNA in MDA-MB-468 cells. We further demonstrate that the process of cell uptake is metabolically driven and that scavenger receptors and caveolae mediated endocytosis play a significant role. Co-localization studies using anti-scavenger receptor antibodies suggest that scavenger receptors are implicated in the mechanism of uptake of CNA modified proteins.

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Article information


Submitted
11 Dec 2019
Accepted
25 Mar 2020
First published
25 Mar 2020

Chem. Commun., 2020, Accepted Manuscript
Article type
Communication

Efficient Cellular Uptake of Click Nucleic Acid Modified Proteins

A. Harguindey, H. Culver, J. Sinha, C. N. Bowman and J. N. Cha, Chem. Commun., 2020, Accepted Manuscript , DOI: 10.1039/C9CC09401F

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