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Programmable RNA methylation and demethylation using PUF RNA binding proteins

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Abstract

New methods to control local RNA methylation are needed to elucidate the function of individual m6A. Here, fusion proteins between the programmable RNA binding protein PUF and the m6A demethylase FTO or METTL14 methyltransferase were designed. FTO-PUFs and METTL14-PUFs showed sequence-specific RNA demethylation and methylation activities, respectively.

Graphical abstract: Programmable RNA methylation and demethylation using PUF RNA binding proteins

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Article information


Submitted
29 Nov 2019
Accepted
23 Dec 2019
First published
25 Dec 2019

Chem. Commun., 2020, Advance Article
Article type
Communication

Programmable RNA methylation and demethylation using PUF RNA binding proteins

K. Shinoda, A. Suda, K. Otonari, S. Futaki and M. Imanishi, Chem. Commun., 2020, Advance Article , DOI: 10.1039/C9CC09298F

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