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Issue 4, 2020
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A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression

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Abstract

Here, we report a novel dual-functional microRNA (miRNA) probe, PA-miRNA, for miRNA target identification and light control of miRNA target expression. PA-miRNA is a miRNA mimic with a 3′-biotin tag linked via a photo-cleavable linker. Using PA-miR-34a, intracellular targets of miR-34a in HeLa cells were isolated and confirmed. Moreover, PA-miR-34a upon transfection into HeLa cells was inactive until light irradiation to break the photo-cleavable linker to release functional miR-34a. We demonstrated that miR-34a target expression as well as miR-34a-promoted cell apoptosis were regulated by PA-miR-34a in a photo-controllable manner.

Graphical abstract: A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression

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Supplementary files

Article information


Submitted
22 Oct 2019
Accepted
06 Dec 2019
First published
06 Dec 2019

Chem. Commun., 2020,56, 627-630
Article type
Communication

A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression

L. Chen, Y. Sun, J. Li and Y. Zhang, Chem. Commun., 2020, 56, 627
DOI: 10.1039/C9CC08277H

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