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Facile fluorescence strategy for sensitive detection of microcystin-LR based on dsDNA-templated copper nanoclusters

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Abstract

We developed a simple, sensitive, low-cost and label-free method to detect microcystin-leucine-arginine (MC-LR) using double-strand DNA-templated copper nanoclusters (dsDNA-CuNCs) as fluorescent probes. The dsDNA template was designed and fabricated by a MC-LR aptamer strand and its complementary strand, which facilitated CuNCs formation and recognized target MC-LR specifically with strong affinity. The formed fluorescent sensing probe of dsDNA-CuNCs exhibited maximum emission wavelength at 575 nm. Upon addition of target MC-LR into dsDNA-CuNCs, fluorescence was quenched considerably due to the high affinity between MC-LR and the aptamer strand, which indicated a conformational change of the dsDNA-CuNCs probe from the dsDNA structure to single-stranded DNA. Then, the change in fluorescence intensity was used to monitor the MC-LR concentration from 0.01 to 1000 μg L−1 with a limit of detection of 4.8 ng L−1. Compared with previous reports, this method did not require design of a complex DNA sequence, fluorescence dye labels or sophisticated experimental techniques. Moreover, the proposed sensing system could be applicable for detection of target MC-LR in real-water samples.

Graphical abstract: Facile fluorescence strategy for sensitive detection of microcystin-LR based on dsDNA-templated copper nanoclusters

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Supplementary files

Article information


Submitted
17 Oct 2019
Accepted
28 Feb 2020
First published
03 Mar 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

Facile fluorescence strategy for sensitive detection of microcystin-LR based on dsDNA-templated copper nanoclusters

Y. Zhang, Y. Lai, X. Teng, S. Pu, Z. Yang, P. Pang, H. Wang, C. Yang, W. Yang and C. J. Barrow, Anal. Methods, 2020, Advance Article , DOI: 10.1039/C9AY02250C

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