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An Ag–TiO2–reduced graphene oxide hybrid film for electrochemical detection of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker

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Abstract

In the present work, a notably sensitive and selective electrochemical 8-OHdG sensor based on an Ag–TiO2–reduced graphene oxide (rGO) hybrid nanomaterial modified screen-printed electrode (SPE) was developed for 8-hydroxy-2′-deoxyguanosine (8-OHdG) determination, which is a significant DNA biomarker because it is one of the main products of oxidative DNA damage. The amperometric responses of the fabricated sensor (Ag–TiO2–rGO/SPE) were measured via monitoring the oxidation current of 8-OHdG by using the differential pulse voltammetry (DPV) technique. The effects of the rGO : Ag–TiO2 ratio, amount of Ag–TiO2–rGO on the electrode surface, electrolyte pH, and possible interfering substances on the current response of Ag–TiO2–rGO/SPE were investigated in detail. Under optimized conditions, the current signals linearly correlated with the concentration of 8-OHdG in the range of 0.05–25 μM (R2 = 0.9967) with a detection limit of 10 nM. Furthermore, Ag–TiO2–rGO/SPE was successfully tested to determine the 8-OHdG level in a human urine sample (only in a volume of 50 μL) with satisfactory recovery.

Graphical abstract: An Ag–TiO2–reduced graphene oxide hybrid film for electrochemical detection of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker

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Article information


Submitted
08 Oct 2019
Accepted
12 Dec 2019
First published
13 Dec 2019

Anal. Methods, 2020, Advance Article
Article type
Paper

An Ag–TiO2–reduced graphene oxide hybrid film for electrochemical detection of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker

A. Jirjees Dhulkefl, K. Atacan, S. Z. Bas and M. Ozmen, Anal. Methods, 2020, Advance Article , DOI: 10.1039/C9AY02175B

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