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Issue 44, 2020
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Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes

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Abstract

A variety of polymeric scaffolds with the ability to control cell detachment has been created for cell culture using stimuli-responsive polymers. However, the widely studied and commonly used thermo-responsive polymeric substrates always affect the properties of the cultured cells due to the temperature stimulus. Here, we present a different stimuli-responsive approach based on poly(3-acrylamidopropyl)trimethylammonium chloride) (poly(APTAC)) brushes with homogeneously embedded superparamagnetic iron oxide nanoparticles (SPIONs). Neuroblastoma cell detachment was triggered by an external magnetic field, enabling a non-invasive process of controlled transfer into a new place without additional mechanical scratching and chemical/biochemical compound treatment. Hybrid scaffolds obtained in simultaneous surface-initiated atom transfer radical polymerization (SI-ATRP) were characterized by atomic force microscopy (AFM) working in the magnetic mode, secondary ion mass spectrometry (SIMS), and X-ray photoelectron spectroscopy (XPS) to confirm the magnetic properties and chemical structure. Moreover, neuroblastoma cells were cultured and characterized before and after exposure to a neodymium magnet. Controlled cell transfer triggered by a magnetic field is presented here as well.

Graphical abstract: Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes

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Supplementary files

Article information


Submitted
30 Jul 2020
Accepted
11 Oct 2020
First published
21 Oct 2020

This article is Open Access

J. Mater. Chem. B, 2020,8, 10172-10181
Article type
Paper

Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes

W. Górka-Kumik, P. Garbacz, D. Lachowicz, P. Dąbczyński, S. Zapotoczny and M. Szuwarzyński, J. Mater. Chem. B, 2020, 8, 10172
DOI: 10.1039/D0TB01853H

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