Micro cell vesicle technology (mCVT): a novel hybrid system of gene delivery for hard-to-transfect (HTT) cells

Yi-Hsuan Ou; Shui Zou; Wei Jiang Goh; Suet Yen Chong; Gopalakrishnan Venkatesan; Matthias G. Wacker; Gerrit Storm; Jiong-Wei Wang; Bertrand Czarny; Giorgia Pastorin; Esther C. Y. Woon

doi:10.1039/D0NR03784B

Micro cell vesicle technology (mCVT): a novel hybrid system of gene delivery for hard-to-transfect (HTT) cells†

Yi-Hsuan Ou,‡^a Shui Zou,‡^a Wei Jiang Goh,^ab Suet Yen Chong,^cd Gopalakrishnan Venkatesan,^a Matthias G. Wacker,^a Gerrit Storm,^chi Jiong-Wei Wang,

*^cde Bertrand Czarny,

*^f Giorgia Pastorin

*^ab and Esther C. Y. Woon

*^ag

Author affiliations

* Corresponding authors

^a Department of Pharmacy, National University of Singapore, Singapore
E-mail: phapg@nus.edu.sg, dr.estherwoon@gmail.com

^b NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), Singapore

^c Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
E-mail: surwang@nus.edu.sg

^d Cardiovascular Research Institute (CVRI), National University Heart Centre Singapore (NUHCS) and National University Health System (NUHS), Singapore

^e Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore

^f School of Materials, Science and Engineering & Lee Kong Chian School of Medicine (LKCmedicine), Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798
E-mail: bczarny@ntu.edu.sg

^g School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK
E-mail: e.woon@ucl.ac.uk

^h Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands

ⁱ Department of Biomaterials Science and Technology, University of Twente, Enschede, Drienerlolaan 5, 7522 NB Enschede, The Netherlands

Abstract

A hybrid gene delivery platform, micro Cell Vesicle Technology (mCVT), produced from the fusion of plasma membranes and cationic lipids, is presently used to improve the transfection efficiency of hard-to-transfect (HTT) cells. The plasma membrane components of mCVTs impart specificity in cellular uptake and reduce cytotoxicity in the transfection process, while the cationic lipids complex with the genetic material and provide structural integrity to mCVTs.

Nanoscale

Micro cell vesicle technology (mCVT): a novel hybrid system of gene delivery for hard-to-transfect (HTT) cells†

Abstract

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Micro cell vesicle technology (mCVT): a novel hybrid system of gene delivery for hard-to-transfect (HTT) cells

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