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Issue 20, 2020
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In-droplet cell separation based on bipolar dielectrophoretic response to facilitate cellular droplet assays

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Abstract

Precise manipulation of cells within water-in-oil emulsion droplets has the potential to vastly expand the type of cellular assays that can be conducted in droplet-based microfluidics systems. However, achieving such manipulation remains challenging. Here, we present an in-droplet label-free cell separation technology by utilizing different dielectrophoretic responses of two different cell types. Two pairs of angled planar electrodes were utilized to generate positive or negative dielectrophoretic force acting on each cell type, which results in selective in-droplet movement of only one specific cell type at a time. A downstream asymmetric Y-shaped microfluidic junction splits the mother droplet into two daughter droplets, each of which contains only one cell type. The capability of this platform was successfully demonstrated by conducting in-droplet separation from a mixture of Salmonella cells and macrophages, two cell types commonly used as a bacterial pathogenicity analysis model. This technology enable the precise manipulation of cells within droplets, which can be exploited as a critical function in implementing broader ranges of droplet-based microfluidics cellular assays.

Graphical abstract: In-droplet cell separation based on bipolar dielectrophoretic response to facilitate cellular droplet assays

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Supplementary files

Article information


Submitted
12 Jul 2020
Accepted
28 Aug 2020
First published
14 Sep 2020

Lab Chip, 2020,20, 3832-3841
Article type
Paper

In-droplet cell separation based on bipolar dielectrophoretic response to facilitate cellular droplet assays

S. Han, C. Huang and A. Han, Lab Chip, 2020, 20, 3832
DOI: 10.1039/D0LC00710B

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