Jump to main content
Jump to site search

Issue 15, 2020
Previous Article Next Article

A new class of ratiometric small molecule intracellular pH sensors for Raman microscopy

Author affiliations

Abstract

Intracellular pH (pHi) homeostasis is intertwined with a myriad of normal cellular behaviors as well as pathological processes. As such, small molecule probes for the measurement of pHi are invaluable tools for chemical biology, facilitating the study of the role of pH in cellular function and disease. The field of small molecule pHi sensors has traditionally been dominated with probes based on fluorescent scaffolds. In this study, a series of low molecular weight (<260) oligoyne compounds have been developed which exhibit pH sensitive alkyne stretching frequencies (νalkyne) in Raman spectroscopy. The modular design of the compounds enabled tuneability of their pKa(H) through simple structural modification, such that continuous pH sensitivity is achieved over the range 2–10. Alkyne stretching bands reside in the ‘cell-silent’ region of the Raman spectrum (1800–2600 cm−1) and are readily detectable in a cellular environment with subcellular spatial resolution. This enabled the application of a pH sensitive oligoyne compound to the ratiometric sensing of pHi in prostate cancer (PC3) cells in response to drug treatment. We propose that probes based on Alkyne Tag Raman Imaging offer an entirely new platform for the sensing of pHi, complementary to fluorescence microscopy.

Graphical abstract: A new class of ratiometric small molecule intracellular pH sensors for Raman microscopy

Back to tab navigation

Supplementary files

Article information


Submitted
29 Apr 2020
Accepted
26 Jun 2020
First published
02 Jul 2020

This article is Open Access

Analyst, 2020,145, 5289-5298
Article type
Paper

A new class of ratiometric small molecule intracellular pH sensors for Raman microscopy

L. T. Wilson, W. J. Tipping, L. E. Jamieson, C. Wetherill, Z. Henley, K. Faulds, D. Graham, S. P. Mackay and N. C. O. Tomkinson, Analyst, 2020, 145, 5289
DOI: 10.1039/D0AN00865F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements