Hemocompatibility Investigation and Improvement of Near-Infrared persistent luminescence nanoparticle ZnGa2O4:Cr3+ by Surface PEGylation
Although near-infrared persistent luminescence nanoparticles are widely used in optical imaging of tumors and grafted cells, there is no report on the behavior of chromium-doped zinc gallate nanoparticles (ZnGa2O4:Cr3+, ZGC) in contact with blood. In this work, monodisperse ZGC with a size of about 10 nm usually used in bioimaging was synthesized by hydrothermal method. We have evaluated the effect of ZGC on blood in terms of hemolysis, erythrocyte morphology, erythrocyte aggregation, coagulation, thrombosis and complement system activation. We improved ZGC blood compatibility by functionalizing them with hydrophilic polyethylene glycol (PEG) polymers. Experimental results demonstrate that the pristine ZGC at a concentration of 0.5 mg mL-1 induce hemolysis, erythrocyte morphology changes and delayed clotting, whereas no significant difference is observed with PEGylated ZGC (ZGC-PEG). However, neither ZGC nor ZGC-PEG caused thrombosis and inflammatory complement activation, which provides a basic research for in vivo imaging.
- This article is part of the themed collection: 2019 Journal of Materials Chemistry B HOT Papers