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Tetraphenylethene decorated with disulfide-functionalized hyperbranched poly(amido amine)s as metal/organic-solvent-free turn-on AIE probes for biothiols determination

Abstract

The development of “turn-on” fluorescence probes for biological thiols detection has received great attention in these years, because abnormal levels of these mercapto biomolecules are always related to some diseases. In this work, a series of AIE-active polymer probes for biothiol detection were designed and synthesized, which comprised disulfide-functionalized hyperbranched poly(amido amine) (ssHPA) as the hydrophilic matrix, tetraphenylethene (TPE) as the hydrophobic AIE-active fluorogen and disulfide bond as the cleavable functional linkage, defined as TPE-ssHPA. When these polymers were targeted as “turn on” fluorescence probes for the detection of biothiols, no organic solvents or heavy metal ions were needed as the quencher to eliminate background signals because of the relatively rigid dendritic structure. Upon introducing glutathione or cysteine to the aqueous solution of TPE-ssHPA, bright fluorescence occurred because of the collapsing of the dendritic structure, leading to the aggregation of TPE units. Further in-vitro experiments verified that TPE-ssHPA is a qualified probe for biothiol detection in living cells. In addition, this strategy of embedding AIEgens inside the relatively rigid hyperbranched polymer offers an effective solution to “pre-quench” the fluorescence of “turn-on” type AIE-active sensors.

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Publication details

The article was received on 30 Jan 2019, accepted on 12 May 2019 and first published on 13 May 2019


Article type: Paper
DOI: 10.1039/C9TB00214F
J. Mater. Chem. B, 2019, Accepted Manuscript

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    Tetraphenylethene decorated with disulfide-functionalized hyperbranched poly(amido amine)s as metal/organic-solvent-free turn-on AIE probes for biothiols determination

    B. Wang, C. Li, L. Yang, C. Zhang, L. Liu, S. Zhu, Y. Chen and Y. Wang, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C9TB00214F

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