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Immobilization of L-Methioninase on Zirconium Based Metal Organic Framework as Anticancer Agent

Abstract

Searching about new anticancer agent is really important and challenged. Herein, zirconium based metal organic framework (UiO-66-(COOH)) was synthesized and L-methioninase enzyme was purified from wickerhamomyces. New composite (METase@UiO-66) from L-methioninase and UiO-66-(COOH) was prepared. The composite METase@UiO-66 was characterized by scanning electron microscopic analysis and powder X-ray diffraction analysis. Moreover, the effects of pH, temperature and storage stability of the composite were well studied. The interesting results lie in great progress on the stability of the composite rather than free enzyme. The composite was stable at 80°C for around 40 minutes and the composite shows better tolerance to the variation of reaction pH (5-7). Also, the storage stability of METase was improved significantly after immobilization where the composite kept its full activity even after 12 days at 4 °C. Not only immobilization of enzyme onto MOFs was tested but also the anti-cancer activity was investigated. For the first time, the composite was tested for treatment of mice bearing tumor and the results of in vivo studies showed that treatment of mice bearing tumor with the METase@UiO-66 composite caused a marked inhibition of tumor growth. Herein, these advantages of the METase@UiO-66 composite make it easily applied as anticancer agent in the field of cancer treatment.

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Publication details

The article was received on 28 Jan 2019, accepted on 31 Mar 2019 and first published on 08 Apr 2019


Article type: Paper
DOI: 10.1039/C9TB00198K
Citation: J. Mater. Chem. B, 2019, Accepted Manuscript

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    Immobilization of L-Methioninase on Zirconium Based Metal Organic Framework as Anticancer Agent

    R. M. Abdelhameed, A. Hassabo, A. M. Mousa , H. Abdel-Gawad and M. H. Selim, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C9TB00198K

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