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Issue 24, 2019
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A hollow-nanosphere-based microfluidic biosensor for biomonitoring of cardiac troponin I

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Abstract

Cardiovascular diseases (CVDs) are the leading causes of death worldwide. Cardiac troponin I (cTnI) is a cardiac biomarker exploited extensively for early diagnosis of acute myocardial infarction (AMI). We report a highly sensitive microfluidic biosensor for the electrochemical detection of human cTnI in real patient samples. In this device, a patterned mesoporous nickel vanadate hollow-nanosphere modified chitosan (Ch-Ni3V2O8) was integrated with a microfluidic structure. Owing to its excellent redox activity and biocompatibility, larger surface, and tunable oxidation states (V5+, V4+, and V3+), the Ch-Ni3V2O8 matrix was used to functionalize the cardiac antibodies of troponin I (cAb) and amplify the electrochemical readouts. This device offered a high sensitivity of 38.88 μA ng−1 mL−1 cm−2 at a wide range of cTnI concentrations (0.005–100 ng mL−1). A low limit-of-detection of 5 pg mL−1 and high stability, high reproducibility and good selectivity were achieved due to integration of microfluidic elements with a hollow-nanosphere Ch-Ni3V2O8. This microfluidic device can be implemented to detect B-type natriuretic peptide, myoglobin, cardiac troponin C, and cardiac troponin T by functionalizing the specific antibody recognition elements and could be used for real point-of-care applications in biomedicine.

Graphical abstract: A hollow-nanosphere-based microfluidic biosensor for biomonitoring of cardiac troponin I

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Supplementary files

Article information


Submitted
19 Jan 2019
Accepted
05 Apr 2019
First published
13 Apr 2019

J. Mater. Chem. B, 2019,7, 3826-3839
Article type
Paper

A hollow-nanosphere-based microfluidic biosensor for biomonitoring of cardiac troponin I

N. Singh, P. Rai, Md. A. Ali, R. Kumar, A. Sharma, B. D. Malhotra and R. John, J. Mater. Chem. B, 2019, 7, 3826
DOI: 10.1039/C9TB00126C

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