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Issue 45, 2019
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Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels

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Abstract

Super resolution microscopy (SRM) brings the advantages of optical microscopy to the imaging of nanostructured soft matter, and in colloidal microgels, promises to quantify variations of crosslink densities at unprecedented length scales. However, the distribution of all crosslinks does not coincide with that of dye-tagged crosslinks, and density quantification in SRM is not guaranteed due to over/under-counting dye molecules. Here we demonstrate that SRM images of microgels encode reaction rate constants of functional cross linkers, which hold the key to correlating these distributions. Combined with evolution of microgel particle radii, the functional cross linker distributions predict consumption versus time with high fidelity. Using a Bayesian regression approach, we extract reaction rate constants for homo and cross propagation of the functional crosslinker, which should be widely useful for predicting spatial variations in crosslink density of gels.

Graphical abstract: Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels

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Publication details

The article was received on 09 Aug 2019, accepted on 28 Oct 2019 and first published on 28 Oct 2019


Article type: Paper
DOI: 10.1039/C9SM01618J
Soft Matter, 2019,15, 9336-9342

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    Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels

    A. A. Karanastasis, G. S. Kenath, R. Sundararaman and C. K. Ullal, Soft Matter, 2019, 15, 9336
    DOI: 10.1039/C9SM01618J

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