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Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

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Abstract

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps. The whole process is achieved simply by exchanging the solution in a single inlet reservoir and by adjusting the applied voltage at a pair of driving electrodes, thus making this approach particularly well-suited for a broad range of research and clinical applications. Further, the use of BPEs allows the array to be scalable to increase throughput. Specific innovations reported here include the incorporation of a leak channel to balance competing flow paths, the use of ‘split BPEs’ to create a distinct recapture and electrical lysis point within the reaction chamber, and the dual purposing of an ionic liquid as an immiscible phase to seal the chambers and as a conductive medium to permit electrical lysis at the split BPEs.

Graphical abstract: Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

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Publication details

The article was received on 27 Oct 2018, accepted on 25 Nov 2018 and first published on 26 Nov 2018


Article type: Edge Article
DOI: 10.1039/C8SC04804E
Citation: Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
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    Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

    M. Li and R. K. Anand, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C8SC04804E

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