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Identification of a common recognition center for a photoactive non-steroidal antiinflammatory drug in serum albumins of different species

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Abstract

The non-steroidal anti-inflammatory drug (S)-carprofen (CPF) has been used as a photoactive probe to investigate the possible existence of a common recognition center in serum albumins (SAs) of different species. The methodology involves irradiation of the CPF/SA complexes, coupled with gel filtration chromatography or proteomic analysis of the photolysates, docking and molecular dynamics simulations. Photolysis of CPF/SA complexes at λ = 320 nm, and gel filtration chromatography, revealed that the protein fraction still contained the drug fluorophore, in agreement with covalent attachment of the photogenerated radical intermediate CBZ˙ to SAs. After trypsin digestion and ESI-MS/MS, the incorporation of CBZ˙ was detected at several positions in the different albumins. Remarkably, modifications at the IB/IIIA interface were observed in all cases (Tyr452 in HSA, RbSA and RtSA and Tyr451 in BSA, PSA and SSA). The molecular basis of this common recognition, studied by docking and molecular dynamics simulation studies on the corresponding non-covalent complexes, corroborated the experimentally observed covalent modifications. Our computational studies also revealed that the previously reported displacement of CPF by (S)-ibuprofen, a site II specific drug, would be due to an allosteric effect in site II, rather than a direct molecular displacement, as expected.

Graphical abstract: Identification of a common recognition center for a photoactive non-steroidal antiinflammatory drug in serum albumins of different species

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Publication details

The article was received on 27 Sep 2018, accepted on 17 Nov 2018 and first published on 19 Nov 2018


Article type: Research Article
DOI: 10.1039/C8QO01045E
Citation: Org. Chem. Front., 2019, Advance Article
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    Identification of a common recognition center for a photoactive non-steroidal antiinflammatory drug in serum albumins of different species

    O. Molins-Molina, E. Lence, D. Limones-Herrero, C. González-Bello, M. A. Miranda and M. C. Jiménez, Org. Chem. Front., 2019, Advance Article , DOI: 10.1039/C8QO01045E

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