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An alternative modular ‘click-SNAr-click’ approach to develop subcellular localised fluorescent probes to image mobile Zn2+

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Abstract

Zn2+ is involved in a number of biological processes and its wide-ranging roles at the subcellular level, especially in specific organelles, have not yet been fully established due to a lack of tools to image it effectively. We report a new and efficient modular double ‘click’ approach towards a range of sub-cellular localised probes for mobile zinc. Through this methodology, endoplasmic reticulum, mitochondria and lysosome localised probes were successfully prepared which show good fluorescence responses to mobile Zn2+ in vitro and in cellulo whilst a non-targeting probe was synthesized as a control. The methodology appears to have wide-utility for the generation of sub-cellular localised probes by incorporating specific organelle targeting vectors for mobile Zn2+ imaging.

Graphical abstract: An alternative modular ‘click-SNAr-click’ approach to develop subcellular localised fluorescent probes to image mobile Zn2+

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Publication details

The article was received on 23 Aug 2019, accepted on 08 Oct 2019 and first published on 10 Oct 2019


Article type: Paper
DOI: 10.1039/C9OB01855G
Org. Biomol. Chem., 2019, Advance Article

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    An alternative modular ‘click-SNAr-click’ approach to develop subcellular localised fluorescent probes to image mobile Zn2+

    L. Fang, G. Trigiante, R. Crespo-Otero, M. P. Philpott, C. R. Jones and M. Watkinson, Org. Biomol. Chem., 2019, Advance Article , DOI: 10.1039/C9OB01855G

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