Issue 35, 2019

Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

Abstract

A modified nucleoside triphosphate bearing two modifications based on a 2′-deoxy-2′-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2′F-ANA UTP) was synthesized. This nucleotide analog could enzymatically be incorporated into DNA oligonucleotides by primer extension and reverse transcribed to unmodified DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.

Graphical abstract: Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

Supplementary files

Article information

Article type
Communication
Submitted
08 Jul 2019
Accepted
19 Aug 2019
First published
19 Aug 2019

Org. Biomol. Chem., 2019,17, 8083-8087

Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

F. Levi-Acobas, A. Katolik, P. Röthlisberger, T. Cokelaer, I. Sarac, M. J. Damha, C. J. Leumann and M. Hollenstein, Org. Biomol. Chem., 2019, 17, 8083 DOI: 10.1039/C9OB01515A

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