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The development of a novel biosensor based on gold nanocages/graphene oxide–chitosan modified acetylcholinesterase for organophosphorus pesticide detection

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Abstract

Based on the inhibition of acetylcholinesterase (AChE), a novel biosensor, namely gold nanocage/graphene oxide–chitosan nanocomposite-modified screen-printed carbon electrode was prepared for chlorpyrifos detection. Particularly, chitosan (CS) functionalized graphene oxide (GO) had a large specific surface area, good dispersibility and adhesion, so we used them to load more gold nanocages (Au NCs). The Au NCs further extended the surface area and promoted electron transfer due to their unique cage structure, thus amplifying the current signal intensity signal of the biosensor. The as-prepared biosensor exhibited good electrocatalytic capacity for the oxidation of enzymatically produced thiocholine and was capable of detecting chlorpyrifos concentrations as low as 3 ng L−1. The inhibition of AChE by chlorpyrifos was proportional to its concentration in the range of 0.01 μg L−1 to 500 μg L−1. Our results indicated that AChE/Au NCs/GO–CS/SPCE was an effective and biocompatible electrode for detecting low concentrations of chlorpyrifos. In addition, the AChE/Au NCs/GO–CS biosensors showed good stability and repeatability, thus providing a promising tool for the detection of organophosphorus pesticides.

Graphical abstract: The development of a novel biosensor based on gold nanocages/graphene oxide–chitosan modified acetylcholinesterase for organophosphorus pesticide detection

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Publication details

The article was received on 17 May 2019, accepted on 01 Aug 2019 and first published on 01 Aug 2019


Article type: Paper
DOI: 10.1039/C9NJ02556A
New J. Chem., 2019, Advance Article

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    The development of a novel biosensor based on gold nanocages/graphene oxide–chitosan modified acetylcholinesterase for organophosphorus pesticide detection

    Y. Yao, G. Wang, G. Chu, X. An, Y. Guo and X. Sun, New J. Chem., 2019, Advance Article , DOI: 10.1039/C9NJ02556A

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