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Behavior of α, β tubulin in DMSO-containing electrolytes

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Abstract

α, β-tubulin is a cytoskeletal protein that forms cylindrical structures termed microtubules, which are crucial to the cell for a variety of roles. Microtubules are frequently modelled as one-dimensional bionanowires that act as ion transporters in the cell. In this work, we used dynamic light scattering (DLS) to measure the hydrodynamic diameter of tubulin in the presence of a polar aprotic co-solvent. We found that the hydrodynamic diameter increased with increasing DMSO volume fraction, almost doubling at 20% DMSO. To evaluate if this was due to an enlarged solvation shell, we performed reference interaction site model (RISM) simulations and found that the extent of solvation was unchanged. Using fluorescence microscopy, we then showed that tubulin was polymerization competent in the presence of colchicine, and thus inferred the presence of oligomers in the presence of DMSO, which points to its mechanism of action as a microtubule polymerization enhancing agent. Tubulin oligomers are known to form when microtubules depolymerize and are controversially implicated in microtubule polymerization. We show that DLS may be used to monitor early-state microtubule polymerization and is a viable alternative to fluorescence and electron microscopy-based methods. Our findings showing that DMSO causes tubulin oligomerization are thus of critical importance, both for creating bio-inspired nanotechnology and determining its biophysical roles in the cell.

Graphical abstract: Behavior of α, β tubulin in DMSO-containing electrolytes

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Publication details

The article was received on 20 Jan 2019, accepted on 18 Jun 2019 and first published on 19 Jun 2019


Article type: Communication
DOI: 10.1039/C9NA00035F
Nanoscale Adv., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
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    Behavior of α, β tubulin in DMSO-containing electrolytes

    A. P. Kalra, P. Kar, J. Preto, V. Rezania, A. Dogariu, J. D. Lewis, J. A. Tuszynski and K. Shankar, Nanoscale Adv., 2019, Advance Article , DOI: 10.1039/C9NA00035F

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