Jump to main content
Jump to site search


Unraveling the photoactive annihilation mechanism of nanostructures as effective green tools for eradicating the proliferation of the phytopathogenic bacterium Pseudomonas syringae

Abstract

The infectious proliferation of phytopathogenic microorganisms depends on a complex sequence of biological events involving host defense, environmental conditions, chemical and physical interactions between the surface of a plant and microorganisms, which in numerous cases display resistance to conventional microbicides. Among these microorganisms, Pseudomonas syringe (P. syringe) is a gram-negative bacterium that attacks wounded parts of plants before invading healthy tissues. In order to control P. syringe, considering it as a phytopathogenic model, an effective method featuring silver nanoparticles (AgNPs) functionalized on titanate nanotubes (Nts) used as photoactive antibacterial agents was investigated to understand the effective annihilation photoactive mechanism. The high dispersion of AgNPs all over the Nts boosted the charge carrier separation by generating reactive oxygen species (ROS), under visible-light, which stressed bacteria and enhanced the biocide effect by quickly preventing the rod-shaped P. syringe bacterium from proliferating. Biological transmission and scanning electron microscopies revealed damaged P. syringe cells that underwent the formation of outer membrane vesicles, caused by the photo-assisted annihilation, which is considered as an indication of critical defense mechanism. The unusual synergistic properties of the Nts, their low cost, and practical synthesis set these nanocomposites as promising green tools that can positively swiftly photokill P. syringae within 30 min.

Back to tab navigation

Publication details

The article was received on 26 Oct 2018, accepted on 14 Apr 2019 and first published on 15 Apr 2019


Article type: Paper
DOI: 10.1039/C8NA00307F
Citation: Nanoscale Adv., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Unraveling the photoactive annihilation mechanism of nanostructures as effective green tools for eradicating the proliferation of the phytopathogenic bacterium Pseudomonas syringae

    S. Casas-Flores, R. B. Domínguez-Espíndola, R. C. Solis, A. A. Patron and V. Rodríguez González, Nanoscale Adv., 2019, Accepted Manuscript , DOI: 10.1039/C8NA00307F

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements