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Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels

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Abstract

We report an additive-free method to lyse bacteria and extract nucleic acids and protein using a traveling surface acoustic wave (TSAW) coupled to a microfluidic device. We characterize the effects of the TSAW on E. coli by measuring the viability of cells exposed to the sound waves and find that about 90% are dead. In addition, we measure the protein and nucleic acids released from the cells and show that we recover about 20% of the total material. The lysis method should work for all types of bacteria. These results demonstrate the feasibility of using TSAW to lyse bacteria in a manner that is independent of the type of bacteria.

Graphical abstract: Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels

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Publication details

The article was received on 08 Jul 2019, accepted on 30 Oct 2019 and first published on 30 Oct 2019


Article type: Paper
DOI: 10.1039/C9LC00656G
Lab Chip, 2019, Advance Article

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    Rapid additive-free bacteria lysis using traveling surface acoustic waves in microfluidic channels

    H. Lu, K. Mutafopulos, J. A. Heyman, P. Spink, L. Shen, C. Wang, T. Franke and D. A. Weitz, Lab Chip, 2019, Advance Article , DOI: 10.1039/C9LC00656G

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