Effect of Ganoderma atrum polysaccharide against pancreatic damage in streptozotocin-induced diabetic mice
This study aimed to explore the role of Ganoderma atrum polysaccharide (PSG-1) on pancreatic damage in streptozotocin (STZ)-induced type 1 diabetes mellitus (T1DM) mice. Results suggested that blood glucose was significantly increased in STZ group in comparison with control group. After 4 weeks treatment with PSG-1 or metformin (MET), blood glucoses in PSG-1 and MET group were apparently lower than STZ group, indicating that PSG-1 triggered hypoglycemic effect in vivo. Moreover, experiments demonstrated that PSG-1 markedly decreased apoptosis of islet cells by inhibiting mitochondrial apoptotic pathway and activating PI3K/Akt survival pathway. PSG-1 also exerted anti-inflammatory effects, as evidences by dramatically reducing levels of IL-1β, TNF-α and INF-γ and restraining TLR4-dependent NF-κB signal pathway. Meanwhile, PSG-1 maintained homeostasis of redox system by increasing activities of anti-oxidant enzymes and reducing malondialdehyde contents in pancreas. Together, these data provided evidence that PSG-1 could be employed as an alternative dietary supplement to ameliorate T1DM.