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Metagenomic analysis revealed beneficial effects of probiotics in improving the composition and function of the gut microbiota in dogs with diarrhoea

Abstract

The aim of the present study was to evaluate the effects of probiotics on the composition and function of the gut microbiota in dogs with diarrhoea. Forty dogs with diarrhoea were randomly allocated to the treatment group or control group. Probiotics, containing Lactobacillus casei Zhang, Lactobacillus plantarum P-8, and Bifdobacterium animalis subsp. lactis V9, was only fed to 20 treated dogs for 60 days. The faecal samples of all dogs at day 0 and day 60 were analyzed using a metagenomic approach. The results showed a significantly higher microbial diversity and obvious change in the structure of gut microbiota in the treatment group. There was also an increase in abundance of some beneficial bacteria in different age dogs, such as Lactobacillus johnsonii (P<0.05), Lactobacillus reuteri (P<0.01), Lactobacillus acidophilus (P<0.05) and Butyricicoccus pullicaecorum (P<0.05), as well as an reduction in abundance of many opportunistic pathogenic bacteria such as Clostridium perfringens (P<0.05) and Stenotrophomonas maltophilia (P<0.05) with supplementation of probiotics. Intriguingly, the correlated networks among some pathogenic bacteria decreased following the administration of the probiotics. Additionally, metagenomic analysis revealed the upregulation of pathways involved in metabolism of amino acids and biosynthesis of secondary metabolites, accompanied with the downregulation of pathways associated with virulence of pathogenic bacteria and cell signaling, suggesting that probiotics could improve the health of dogs with diarrhoea through regulation of the gut microbiota. Our research provides new information relevant to the treatment of diarrhoea in animals and human.

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Publication details

The article was received on 15 Jan 2019, accepted on 09 Apr 2019 and first published on 10 Apr 2019


Article type: Paper
DOI: 10.1039/C9FO00087A
Citation: Food Funct., 2019, Accepted Manuscript

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    Metagenomic analysis revealed beneficial effects of probiotics in improving the composition and function of the gut microbiota in dogs with diarrhoea

    H. Xu, F. Zhao, Q. Hou, W. huang, Y. Liu, H. Zhang and Z. Sun, Food Funct., 2019, Accepted Manuscript , DOI: 10.1039/C9FO00087A

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